GalSeq was created by a team of scientists with a strong experience in Next Generation Sequencing and in Bioinformatics.
As scientist, we know what type of services and analyses researchers need.

The conversion of large amounts of complex data into biologically reliable and informative results, which represents a real challenge for many researchers, is our strength.

WHOLE GENOME Sequencing

Whole genome sequencing (WGS) allows researchers to investigate through the entire genome sequence (coding, non-coding and mtDNA). It can be applied to both Eukaryotes and Prokaryotes, as well as organisms where there is no reference genome sequence available (De novo sequencing), allowing accurate characterization of any species. Human WGS allows discovery of all DNA variants, including those that lie outside the coding regions. Although most of the known disease-causing variants are located in the coding regions and can be detected by exome sequencing, with whole genome sequencing you can also investigate regulatory regions, like promoters, enhancers and silencers where alterations can affect gene activity and protein production, as well as non-coding RNA genes. Whole genome sequencing is also useful to characterize complex structural variations of the genome.
Galseq offers both resequencing service for all organisms where a reference genome is already available, including humans, animals, plants, bacteria, and viruses as well as De novo sequencing service with assembling of novel genomes. Testo Ancora

RNA Sequencing

Transcriptome sequencing is a powerful tool for quantifying transcripts and discovering novel transcripts and gene fusions. Galseq offers strand specific mRNA libraries prep (by selection of poly-A transcripts) as well as total RNA libraries prep with depletion of ribosomal RNA, starting from a wide range of input material, including FFPE and exosomal RNA, with very low input, down to few picograms. Galseq offers a complete RNA-seq data analysis to study:
- gene expression profiles and differential expression analysis
- new transcripts and isoforms
- alternative splicing sites
- fusion genes and structural variants
- point mutations
Small RNA sequencing is largely adopted for gene regulation studies at the transcriptional and post-transcriptional levels. Galseq offers small RNA-seq data analysis to discover novel miRNAs and other small non-coding RNAs and to study the differential expression of all small RNAs.Testo Ancora

MICROBIOME Sequencing

Microbiome sequencing allows researchers to investigate the composition of microbiota in different biological samples. Sequencing of 16S rRNA variable regions for bacteria and Internal Transcribed Spacer (ITS) rRNA for fungi is a well-established amplicon sequencing method that allows taxonomic classification of bacteria and fungi present in a given sample. Galseq offers V1-V2, V3 and V4 16S rRNA as well as ITS1 rRNA sequencing service together with customized bioinformatics analysis.
Galseq developed Microbiome software to visualize microbiome sequencing results and analyses.
All customers are provided with an interactive data report to run their own custom analyses in a user-friendly environment.Testo Ancora

WHOLE EXOME Sequencing

Human exome represents less than 2% of the genome, but contains ~80-90% of known disease-causing variants, making whole-exome sequencing a cost-effective alternative to whole-genome sequencing. Galseq offers human whole-exome sequencing as well as exome-seq for some non-human species (mouse, bovine, zebrafish) and pull-down of custom regions. Galseq offers complete exome data analysis including:
- comparative case/control analysis, such as tumoral vs normal sample with detection of somatic variants and CNA/allelic-imbalance (AI) with CEQer software, an internal developed bioinformatics tool;
- detection of germline variants in family members (Trio analysis).
Galseq recommends a coverage of 80-100x for somatic variants detection and a coverage of 50-60x for germline variants and SNPs detection.Testo Ancora

Ultradeep Sequencing

Ultra deep sequencing consists in sequencing small genomic regions of interest multiple times, typically thousands times. This technique allows detection of rare clonal types, cells or microbes and low-frequency variants comprising as little as 1% of the original sample. To increase the sensitivity and reduce false positives Galseq offers library preparation service with the introduction of unique molecular identifiers (UMI) within the adapter, enabling high-resolution reads and accurate detection of true variants.Testo Ancora

CHIP Sequencing

ChIP-seq combines chromatin immunoprecipitation with DNA sequencing to identify the target sequences of DNA-associated proteins: DNA-bound proteins are immunoprecipitated using a specific antibody and bound DNA is coprecipitated, purified, and sequenced. ChIP-Seq is a powerful discovery tool to identify transcription factors binding sites and epigenetic marks. Galseq offers the entire ChIP-Seq workflow, from library preparation to data analysis. Testo Ancora

Gene Panels

Targeted resequencing allows you to target and sequence only genomic regions of interest, making it a cost-effective tool. Galseq offers enrichment panels with amplicon-based or hybridization-based methods to identify:
- somatic variants associated with the most common solid tumors, including lung, colon, melanoma, gastric cancer, ovarian and breast
- somatic variants associated with hematologic malignancies
- germline variants associated with a predisposition towards cancer
- germline variants associated with hereditary diseases
- germline variants associated with cardiomyopathies and other inherited cardiac conditions
- germline variants associated with thrombotic diseases
Galseq offers the design and sequencing of custom panels according to the researcher's needs. Testo Ancora

ATAC Sequencing

The assay for transposase-accessible chromatin sequencing (ATAC-Seq) is an effective method used to reveal chromatin accessibility at a genome-wide level. By sequencing regions of open chromatin, ATAC-Seq is a powerful epigenetic discovery tool that helps researchers to understand how chromatin packaging affects gene expression. ATAC-seq experiments has been used to understand dynamic gene expression regulation in stem cells, embryonic development, complex diseases, T-cell activation and tumorigenesis. Galseq offers the entire ATAC-Seq workflow, from library preparation to data analysis.

METHYLATION Sequencing

DNA methylation is an epigenetic modification that plays significant roles in regulating gene expression and influences many biological processes such as embryonic development, tissue regeneration and pathogenesis. Next-generation sequencing technologies allow for genome-wide profiling of methylation patterns at a single nucleotide level through bisulfite conversion of DNA to detect unmethylated cytosines. Galseq offers different approaches:
- Whole-genome bisulfite sequencing (WGBS): bisulfite conversion is applied to the whole genome;
- Reduced representation bisulfite sequencing (RRBS): biologically relevant target regions including promoters and CpG islands are enriched through digestion with multiple restriction enzymes, then treated with bisulfite and sequenced;
- Targeted methylation sequencing: known differentially methylated regions are enriched with hybridization-based methods, then bisulfite treated and sequenced;
- Methylated DNA immunoprecipitation (MeDIP): methylated DNA fragments are enriched with 5-methylcytosine (5mC) antibody immunoprecipitation and then sequenced. This method lacks resolution at a single nucleotide level.